Category: 2140-71-8

Alteration of cross-linking selectivity with the 2′-OMe analogue of 2-amino-6-vinylpurine and evaluation of antisense effects was written by Imoto, Shuhei;Hori, Tsuneaki;Hagihara, Shinya;Taniguchi, Yosuke;Sasaki, Shigeki;Nagatsugi, Fumi. And the article was included in Bioorganic & Medicinal Chemistry Letters in 2010.Application of 2140-71-8 This article mentions the following:

We previously reported that oligodeoxynucleotides containing 2-amino-6-vinylpurine (2-AVP) exhibit efficient selective crosslinking to cytosine. In this study, the 2′-OMe nucleoside analog (2) of 2-AVP was designed in order to increase its affinity to RNA and enhance metabolic stability. It has been demonstrated that 2′-OMe oligonucleotides bearing 2 achieve highly selective crosslinking to the thymine base in DNA and show higher antisense effect on luciferase production in cell lysate. In the experiment, the researchers used many compounds, for example, 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8Application of 2140-71-8).

2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. THF (Tetrahydrofuran) is also used as a starting material for the synthesis of poly(tetramethylene ether) glycol (PTMG), etc.Application of 2140-71-8

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Protein-induced B-Z transition of DNA duplex containing a 2-OMe guanosine was written by Jin, Ho-Seong;Kim, Na-Hyun;Choi, Seo-Ree;Oh, Kwang-Im;Lee, Joon-Hwa. And the article was included in Biochemical and Biophysical Research Communications in 2020.Name: 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one This article mentions the following:

Structural transformation of the canonical right-handed helix, B-DNA, to the non-canonical left-handed helix, Z-DNA, can be induced by the Zα domain of the human RNA editing enzyme ADAR1 (hZαADAR1). To characterize the site-specific preferences of binding and structural changes in DNA containing the 2′-O-Me guanosine derivative (mG), titration of the imino proton spectra and chem. shift perturbations were performed on hZαADAR1 upon binding to Z-DNA. The structural transition between B-Z conformation as the changing ratio between DNA and protein showed a binding affinity of the modified DNA onto the Z-DNA binding protein similar to wild-type DNA or RNA. The chem. shift perturbation results showed that the overall structure and environment of the modified DNA revealed DNA-like properties rather than RNA-like characteristics. Moreover, we found evidence for two distinct regimes, “Z-DNA Sensing” and “Modification Sensing”, based on the site-specific chem. shift perturbation between the DNA (or RNA) binding complex and the modified DNA-hZαADAR1 complex. Thus, we propose that modification of the sugar backbone of DNA with 2′-O-Me guanosine promotes the changes in the surrounding α3 helical structural segment as well as the non-perturbed feature of the β-hairpin region. In the experiment, the researchers used many compounds, for example, 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8Name: 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one).

2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.Name: 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

A quantitative systems approach reveals dynamic control of tRNA modifications during cellular stress was written by Chan, Clement T. Y.;Dyavaiah, Madhu;DeMott, Michael S.;Taghizadeh, Koli;Dedon, Peter C.;Begley, Thomas J.. And the article was included in PLoS Genetics in 2010.Application In Synthesis of 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one This article mentions the following:

Decades of study have revealed more than 100 ribonucleoside structures incorporated as post-transcriptional modifications mainly in tRNA and rRNA, yet the larger functional dynamics of this conserved system are unclear. To this end, we developed a highly precise mass spectrometric method to quantify tRNA modifications in Saccharomyces cerevisiae. Our approach revealed several novel biosynthetic pathways for RNA modifications and led to the discovery of signature changes in the spectrum of tRNA modifications in the damage response to mechanistically different toxicants. This is illustrated with the RNA modifications Cm, m⁵C, and m² ₂G, which increase following hydrogen peroxide exposure but decrease or are unaffected by exposure to methylmethane sulfonate, arsenite, and hypochlorite. Cytotoxic hypersensitivity to hydrogen peroxide is conferred by loss of enzymes catalyzing the formation of Cm, m⁵C, and m² ₂G, which demonstrates that tRNA modifications are critical features of the cellular stress response. The results of our study support a general model of dynamic control of tRNA modifications in cellular response pathways and add to the growing repertoire of mechanisms controlling translational responses in cells. In the experiment, the researchers used many compounds, for example, 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8Application In Synthesis of 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one).

2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8) belongs to tetrahydrofuran derivatives. Solid acid catalysis, and the advantages often associated with their use, have been proved equally efficient for the synthesis of tetrahydrofurans or furans. THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.Application In Synthesis of 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Laser desorption/Fourier-transform mass spectrometry for the study of nucleosides, oligosaccharides, and glycosides was written by McCrery, David A.;Gross, Michael L.. And the article was included in Analytica Chimica Acta in 1985.Quality Control of 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one This article mentions the following:

Laser desorption/Fourier-transform mass spectrometry (l.d./F.t.m.s.) was used to investigate the feasibility of desorbing nucleosides, oligosaccharides, and glycosides into the gas phase. Nucleosides were desorbed as (M – H)^– ions or as (M + H)⁺ or, more readily, as alkali metal ion cationized species. Monosaccharides also could be desorbed as (M – H)^–, but not di- or trisaccharides. At higher laser irradiance, very abundant cationized oligosaccharides were observed This was particularly true when the Nd:YAG laser was operated in a non-Q-switched mode to give a longer lasting laser pulse. Ions of nonreducing sugars cleaved at the glycosidic bond, but those of reducing sugars underwent ring cleavage reactions. Various glycosides including quercitrin, rutin, xanthorhamnin, digoxin, digitoxin, and erythromycin were also successfully desorbed as cationized species by using l.d./F.t.m.s. Digoxin and digitoxin (M + Na)⁺ ions fragment different than the (M + H)⁺ ions, presumably because the Na⁺ is primarily affiliated with the sugar moiety, whereas H⁺ is bound on the steroid portion of the mol. In the experiment, the researchers used many compounds, for example, 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8Quality Control of 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one).

2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is water-miscible and has a low viscosity making it a highly versatile solvent used in a variety of industries. Tetrahydrofuran can also be produced, or synthesised, via catalytic hydrogenation of furan. This process involves converting certain sugars into THF by digesting to furfural. An alternative to this method is the catalytic hydrogenation of furan with a nickel catalyst.Quality Control of 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Quantitative measurement of mRNA cap 0 and cap 1 structures by high-performance liquid chromatography was written by Kuo, Kenneth C.;Smith, Christine E.;Shi, Zhixian;Agris, Paul F.;Gehrke, Charles W.. And the article was included in Journal of Chromatography, Biomedical Applications in 1986.HPLC of Formula: 2140-71-8 This article mentions the following:

The use of reversed-phase HPLC is described as a rapid and efficient tool for the separation, identification, and quantitation of caps of mRNA. An ion-exchange enrichment procedure was also developed for the isolation of cap 0 and cap 1 structures from unfractionated RNAs. The recoveries of different caps ranged 83-99%, with a relative standard deviation range of 1.3-4.4%. In this method, caps were released from com. obtained rabbit globin mRNA by nuclease P1 digestion. The products of digestion were treated with alk. phosphatase and separated on an octadecylsilyl column using stepwise or gradient elution. Cap structures and any internal modified nucleosides were identified by their retention times and UV spectra relative to reference compounds The amount of each cap 0 or cap 1 structure was determined by its UV absorbance relative to a known quality of reference compound This method allows the quantitation of ≥0.2 nmol of cap 0 and cap 1 structures. Total UV spectra can be obtained for ≥0.5 nmol of cap. This methodol. permits investigations on viral and eukaryotic mRNA cap biosynthesis and turnover during viral transformation, differentiation, cap synthesis in the cell cycle, etc. In the experiment, the researchers used many compounds, for example, 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8HPLC of Formula: 2140-71-8).

2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.HPLC of Formula: 2140-71-8

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Quantitative measurement of mRNA cap 0 and cap 1 structures by high-performance liquid chromatography was written by Kuo, Kenneth C.;Smith, Christine E.;Shi, Zhixian;Agris, Paul F.;Gehrke, Charles W.. And the article was included in Journal of Chromatography, Biomedical Applications in 1986.HPLC of Formula: 2140-71-8 This article mentions the following:

The use of reversed-phase HPLC is described as a rapid and efficient tool for the separation, identification, and quantitation of caps of mRNA. An ion-exchange enrichment procedure was also developed for the isolation of cap 0 and cap 1 structures from unfractionated RNAs. The recoveries of different caps ranged 83-99%, with a relative standard deviation range of 1.3-4.4%. In this method, caps were released from com. obtained rabbit globin mRNA by nuclease P1 digestion. The products of digestion were treated with alk. phosphatase and separated on an octadecylsilyl column using stepwise or gradient elution. Cap structures and any internal modified nucleosides were identified by their retention times and UV spectra relative to reference compounds The amount of each cap 0 or cap 1 structure was determined by its UV absorbance relative to a known quality of reference compound This method allows the quantitation of ≥0.2 nmol of cap 0 and cap 1 structures. Total UV spectra can be obtained for ≥0.5 nmol of cap. This methodol. permits investigations on viral and eukaryotic mRNA cap biosynthesis and turnover during viral transformation, differentiation, cap synthesis in the cell cycle, etc. In the experiment, the researchers used many compounds, for example, 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8HPLC of Formula: 2140-71-8).

2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.HPLC of Formula: 2140-71-8

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem