Category: 2140-69-4

Surpassing limits of static RNA modification analysis with dynamic NAIL-MS was written by Reichle, Valentin F.;Kaiser, Steffen;Heiss, Matthias;Hagelskamp, Felix;Borland, Kayla;Kellner, Stefanie. And the article was included in Methods (Amsterdam, Netherlands) in 2019.Recommanded Product: 2140-69-4 This article mentions the following:

Ribonucleic acids (RNA) are extensively modified. These modifications are quantified by mass spectrometry (LC-MS/MS) to determine the abundance of a modification under certain conditions or in various genetic backgrounds. With LC-MS/MS the steady state of modifications is determined, and thus we only have a static view of the dynamics of RNA modifications. With nucleic acid isotope labeling coupled mass spectrometry (NAIL-MS) we overcome this limitation and get access to the dynamics of RNA modifications. We describe labeling techniques for E. coli, S. cerevisiae and human cell culture and the current instrumental limitations. We present the power of NAIL-MS but we also outline validation experiments, which are necessary for correct data interpretation. As an example, we apply NAIL-MS to study the demethylation of adenine and cytidine, which are methylated by the damaging agent methyl-methanesulfonate in E. coli. With NAIL-MS we exclude the concurrent processes for removal of RNA methylation, namely RNA degradation, turnover and dilution We use our tool to study the speed and efficiency of 1-methyladenosine and 3-methylcytidine demethylation. We further outline current limitations of NAIL-MS but also potential future uses for e.g. relative quantification of tRNA isoacceptor abundances. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4Recommanded Product: 2140-69-4).

1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.Recommanded Product: 2140-69-4

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Transient conductivity of 1,3-dimethyluracil, uridine and 3-methyluridine in aqueous solution following 20-ns laser excitation at 248 nm was written by Goerner, Helmut;Currell, Leslie J.. And the article was included in Radiation Physics and Chemistry in 1996.Category: tetrahydrofurans This article mentions the following:

Uridine, 3-methyluridine and 1,3-dimethyluracil in aqueous solution were studied by time-resolved conductimetry after excitation at 248 nm by 20-ns laser pulses. The conductivity signal increases to the maximum value at the pulse end (Δκ^m) and decreases then with time, depending on the saturating gas (Ar, N₂O, O₂ or MeCl) and pH. The Δκ^m signal is suggested to originate from hydrated electrons (e_aq^–) and protons, the latter resulting from radical cations after rapid reaction with water. Biphotonic photoionization occurs in the whole pH range 3-11 with a quantum yield of 0.016 or smaller for laser intensities of ≤8 MW/cm². The reaction of e_aq^– with uncharged bases in Ar-saturated solution at pH 5-8 generates radical anions which are subsequently protonated. The neutralization reaction kinetics of the uracil derivatives depend essentially on the transient proton concentration and lead to the disappearance of most of the conductivity (>90%) within a few microseconds or less in neutral or acidic solution, resp. For 3-methyluridine and uridine after neutralization, the presence of a long-lived species with acidic properties was observed (in small yield) upon biphotonic (but not monophotonic) excitation. The time-resolved conductivity pattern in the alk. pH range is different for each of the three pyrimidines, depending essentially on the generation or consumption of OH^– in the radical termination reactions. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4Category: tetrahydrofurans).

1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). Commercial tetrahydrofuran contains substantial water that must be removed for sensitive operations, e.g. those involving organometallic compounds. Although tetrahydrofuran is traditionally dried by distillation from an aggressive desiccant, molecular sieves are superior.Category: tetrahydrofurans

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Comprehensive profiling of ribonucleosides modification by affinity zirconium oxide-silica composite monolithic column online solid-phase microextraction – Mass spectrometry analysis was written by Jiang, Han-Peng;Chu, Jie-Mei;Lan, Meng-Dan;Liu, Ping;Yang, Na;Zheng, Fang;Yuan, Bi-Feng;Feng, Yu-Qi. And the article was included in Journal of Chromatography A in 2016.SDS of cas: 2140-69-4 This article mentions the following:

More than 140 modified ribonucleosides have been identified in RNA. Determination of endogenous modified ribonucleosides in biol. fluids may serve as non-invasive disease diagnostic strategy. However, detection of the modified ribonucleosides in biol. fluids is challenging, especially for the low abundant modified ribonucleosides due to the serious matrix interferences of biol. fluids. Here, we developed a facile preparation strategy and successfully synthesized zirconium oxide-silica (ZrO₂/SiO₂) composite capillary monolithic column that exhibited excellent performance for the selective enrichment of cis-diol-containing compounds Compared with the boronate-based affinity monolith, the ZrO₂/SiO₂ monolith showed ∼2 orders of magnitude higher extraction capacity and can be used under physiol. pH (pH 6.5-7.5). Using the prepared ZrO₂/SiO₂ composite monolith as the trapping column and reversed-phase C18 column as the anal. column, we further established an online solid-phase microextraction (SPME) in combination with liquid chromatog.-mass spectrometry (online SPME-LC-MS/MS) anal. for the comprehensive profiling of ribonucleosides modification in human urine. Our results showed that 68 cis-diol-containing ribosylated compounds were identified in human urine, which is, to the best of our knowledge, the highest numbers of cis-diol-containing compounds were determined in a single anal. It is worth noting that four modified ribonucleosides were discovered in the human urine for the first time. In addition, the quantification results from the pooled urine samples showed that compared to healthy controls, the contents of sixteen ribose conjugates in the urine of gastric cancer, eleven in esophagus cancer and seven in lymphoma increased more than two folds. Among these ribose conjugates, four ribose conjugates increased more than two folds in both gastric cancer and esophagus cancer; three ribose conjugates increased more than two folds in both gastric cancer and lymphoma; one ribose conjugate increased more than two folds in both esophagus cancer and lymphoma. The developed anal. method provides a good platform to study the modified ribonucleosides in human body fluids. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4SDS of cas: 2140-69-4).

1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is water-miscible and has a low viscosity making it a highly versatile solvent used in a variety of industries. Oxidations have also proved to be valuable and efficient approaches to chiral tetrahydrofuran derivatives.SDS of cas: 2140-69-4

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Metabolic significance of bisphenol A-induced oxidative stress in rat urine measured by liquid chromatography-mass spectrometry was written by Cho, Sung-Hee;Choi, Man Ho;Kwon, Oh Seung;Lee, Won-Yong;Chung, Bong Chul. And the article was included in Journal of Applied Toxicology in 2009.Formula: C10H14N2O6 This article mentions the following:

Modified nucleosides are formed by DNA repair as a result of oxidative DNA damage and post-transcriptionally modified tRNA in cells. In the present study, the profiling of 14 nucleosides was investigated in rat urine to evaluate bisphenol A (BPA)-induced oxidative stress after i.p. injecting rats with 0, 10, or 50 mg kg^-1 per day of BPA for 4 consecutive days. The urinary concentrations of individual nucleosides were measured by liquid chromatog.-tandem mass spectrometry combined with column switching online extraction Increased levels of 5-hydroxymethyl-2′-deoxyuridine (P < 0.01 on first, P < 0.005 on second, P < 0.001 on third, and P < 0.01 on fourth day) and 8-hydroxy-2′-deoxyguanosine (P < 0.005 on second, P < 0.001 on third, and P < 0.001 on fourth day) were found. Also, the patterns of urinary nucleosides in 3 dosage groups (control, BPA1, BPA2) were significantly different. Statistical significance was observed between BPA1 (5-hydroxymethyl-2′-deoxyuridine, P < 0.05; 8-hydroxy-2′-deoxyguanosine, P < 0.005) and BPA2 (5-hydroxymethyl-2′-deoxyurindine, P < 0.005; 8-hydroxy-2′-deoxyguanosine, P < 0.001) during the treatment period. Supervised anal. with partial least-squares-discrimination anal. led to discrimination between the 3 dosage groups. Quant. alterations showed the metabolic trajectories responsible for physiol. responses. The described methods could be used to evaluate and monitor BPA-induced oxidatives stress early after exposure. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4Formula: C10H14N2O6).

1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is water-miscible and has a low viscosity making it a highly versatile solvent used in a variety of industries. Commercial tetrahydrofuran contains substantial water that must be removed for sensitive operations, e.g. those involving organometallic compounds. Although tetrahydrofuran is traditionally dried by distillation from an aggressive desiccant, molecular sieves are superior.Formula: C10H14N2O6

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Normal and modified nucleosides in urine as potential tumor markers determined by MEKC and HPLC was written by Xu, Guowang;Liebich, Hartmut. And the article was included in American Clinical Laboratory in 2001.COA of Formula: C10H14N2O6 This article mentions the following:

A study was conducted which give further credence to previous observations that patterns of urinary excretion of modified nucleosides may serve as a useful diagnostic tool for malignant disease, especially when the pattern recognition method is used together with micellar electrokinetic capillary chromatog. or high-performance liquid chromatog. (HPLC). A major advantage for using the nucleosides as possible biomarkers lies in the MEKC or HPLC method. All of them can be accurately and quickly measured per individual urine and/or serum sample in one MEKC or HPLC run. Although further studies are needed, the use of modified nucleosides in combination with other biomarkers may have potential application as an adjunct to the clin. assessment of cancer patients. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4COA of Formula: C10H14N2O6).

1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives. Tetrahydrofuran and dihydrofuran form the basic structural unit of many naturally occurring scaffolds like gambieric acid A and ciguatoxin, goniocin, and some biologically active molecules. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.COA of Formula: C10H14N2O6

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

A urinary metabonomics study on biochemical changes in yeast-induced pyrexia rats: A new approach to elucidating the biochemical basis of the febrile response was written by Gao, Xiaoyan;Guo, Mingxing;Zhao, Baosheng;Peng, Long;Su, Jiankun;Bai, Xu;Li, Jun;Qiao, Yanjiang. And the article was included in Chemico-Biological Interactions in 2013.Computed Properties of C10H14N2O6 This article mentions the following:

Fever is a prominent feature of many diseases, such as infection, inflammation and trauma. In the clinic, fever can be easily judged by measuring the body temperature; however, the pathogenesis of fever is still not fully understood. A febrile response is a systemic pathol. process that can cause metabolic disorders. Metabonomics can provide powerful tools to reveal the pathol. mechanisms for such a systemic disease. Thus, to reveal subtle metabolic changes under the condition of fever and to explore its mechanism, an ultra performance liquid chromatog. coupled with a quadrupole time-of-flight mass spectrometry metabonomics approach was employed to investigate the urine biochem. characteristics of yeast-induced pyrexia rats. The acquired data were subjected to principal component anal. for differentiating the pyrexia rats from the control rats. Potential biomarkers were screened by using orthogonal partial least-squares-discriminant anal. and were identified by accurate mass, database, and MS/MS fragment information obtained from the MS^E technique. Sixteen metabolites in rat urine were identified as potential biomarkers. The relative intensities of the 15 potential biomarkers were calculated The thermoregulatory circuitry of “endogenous pyrogen (EP) ↑-hypothalamus Na⁺/Ca²⁺-cAMP↑” was partially confirmed in this study. The results suggested that UPLC/MS-based metabolic profiling of rat urine identifies impaired tryptophan metabolism as the mechanism of yeast-induced fever. This research provided informative data that the impaired tryptophan metabolism might be one of the important reasons in elucidating the biochem. basis of the febrile response. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4Computed Properties of C10H14N2O6).

1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives.Tetrahydrofuran has many industry uses as a solvent including in natural and synthetic resins, high polymers, fat oils, rubber, polymer. Tetrahydrofuran (THF) is primarily used as a precursor to polymers including for surface coating, adhesives, and printing inks.Computed Properties of C10H14N2O6

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Divergence in the metabolome between natural aging and Alzheimer’s disease was written by Hunsberger, Holly C.;Greenwood, Bennett P.;Tolstikov, Vladimir;Narain, Niven R.;Kiebish, Michael A.;Denny, Christine Ann. And the article was included in Scientific Reports in 2020.Recommanded Product: 2140-69-4 This article mentions the following:

Alzheimer’s disease (AD) is a progressive and debilitating neurodegenerative disorder and one of the leading causes of death in the United States. Although amyloid plaques and fibrillary tangles are hallmarks of AD, research suggests that pathol. associated with AD often begins 20 or more years before symptoms appear. Therefore, it is essential to identify early-stage biomarkers in those at risk for AD and age-related cognitive decline (ARCD) in order to develop preventative treatments. Here, we used an untargeted metabolomics anal. to define system-level alterations following cognitive decline in aged and APP/PS1 (AD) mice. At 6, 12, and 24 mo of age, both control (Ctrl) and AD mice were tested in a 3-shock contextual fear conditioning (CFC) paradigm to assess memory decline. AD mice exhibited memory deficits across age and these memory deficits were also seen in naturally aged mice. Prefrontal cortex (PFC), hippocampus (HPC), and spleen were then collected and analyzed for metabolomic alterations. A number of significant pathways were altered between Ctrl and AD mice and naturally aged mice. By identifying systems-level alterations following ARCD and AD, these data could provide insights into disease mechanisms and advance the development of biomarker panels. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4Recommanded Product: 2140-69-4).

1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). Tetrahydrofuran (THF) is primarily used as a precursor to polymers including for surface coating, adhesives, and printing inks.Recommanded Product: 2140-69-4

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem

Divergence in the metabolome between natural aging and Alzheimer’s disease was written by Hunsberger, Holly C.;Greenwood, Bennett P.;Tolstikov, Vladimir;Narain, Niven R.;Kiebish, Michael A.;Denny, Christine Ann. And the article was included in Scientific Reports in 2020.Recommanded Product: 2140-69-4 This article mentions the following:

Alzheimer’s disease (AD) is a progressive and debilitating neurodegenerative disorder and one of the leading causes of death in the United States. Although amyloid plaques and fibrillary tangles are hallmarks of AD, research suggests that pathol. associated with AD often begins 20 or more years before symptoms appear. Therefore, it is essential to identify early-stage biomarkers in those at risk for AD and age-related cognitive decline (ARCD) in order to develop preventative treatments. Here, we used an untargeted metabolomics anal. to define system-level alterations following cognitive decline in aged and APP/PS1 (AD) mice. At 6, 12, and 24 mo of age, both control (Ctrl) and AD mice were tested in a 3-shock contextual fear conditioning (CFC) paradigm to assess memory decline. AD mice exhibited memory deficits across age and these memory deficits were also seen in naturally aged mice. Prefrontal cortex (PFC), hippocampus (HPC), and spleen were then collected and analyzed for metabolomic alterations. A number of significant pathways were altered between Ctrl and AD mice and naturally aged mice. By identifying systems-level alterations following ARCD and AD, these data could provide insights into disease mechanisms and advance the development of biomarker panels. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4Recommanded Product: 2140-69-4).

1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). Tetrahydrofuran (THF) is primarily used as a precursor to polymers including for surface coating, adhesives, and printing inks.Recommanded Product: 2140-69-4

Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem