Category: 18423-43-3

Identification of DHODH as a therapeutic target in small cell lung cancer was written by Li, Leanne; Ng, Sheng Rong; Colon, Caterina I.; Drapkin, Benjamin J.; Hsu, Peggy P.; Li, Zhaoqi; Nabel, Christopher S.; Lewis, Caroline A.; Romero, Rodrigo; Mercer, Kim L.; Bhutkar, Arjun; Phat, Sarah; Myers, David T.; Muzumdar, Mandar Deepak; Westcott, Peter M. K.; Beytagh, Mary Clare; Farago, Anna F.; Vander Heiden, Matthew G.; Dyson, Nicholas J.; Jacks, Tyler. And the article was included in Science Translational Medicine in 2019.Reference of 18423-43-3 The following contents are mentioned in the article:

Small cell lung cancer (SCLC) is an aggressive lung cancer subtype with extremely poor prognosis. No targetable genetic driver events have been identified, and the treatment landscape for this disease has remained nearly unchanged for over 30 years. Here, we have taken a CRISPR-based screening approach to identify genetic vulnerabilities in SCLC that may serve as potential therapeutic targets. We used a single-guide RNA (sgRNA) library targeting ∼5000 genes deemed to encode “”druggable”” proteins to perform loss-of-function genetic screens in a panel ofcell lines derived from autochthonous genetically engineered mouse models (GEMMs) of SCLC, lung adenocarcinoma (LUAD), and pancreatic ductal adenocarcinoma (PDAC). Cross-cancer analyses allowed us to identify SCLC-selective vulnerabilities. In particular, we observed enhanced sensitivity of SCLC cells toward disruption of the pyrimidine biosynthesis pathway. Pharmacol. inhibition of dihydroorotate dehydrogenase (DHODH), a key enzyme in this pathway, reduced the viability of SCLC cells in vitro and strongly suppressed SCLC tumor growth in human patient-derived xenograft (PDX) models and in an autochthonous mouse model. These results indicate that DHODH inhibition may be an approach to treat SCLC. This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3Reference of 18423-43-3).

Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF), or oxolane, is mainly used as a precursor to polymers. Being polar and having a wide liquid range, THF is a versatile solvent. THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.Reference of 18423-43-3

18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3

Effect of various phosphate-containing substances on the rate of vanadyl sulfate oxidation by potassium permanganate was written by Kriss, E. E.; Yatsimirskii, K. B.. And the article was included in Zhurnal Neorganicheskoi Khimii in 1969.Recommanded Product: Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt The following contents are mentioned in the article:

VOSO4 oxidation by KMnO4 in H2SO4 solution was studied by stopped-flow method. The oxidation was accomplished within few sec. and proceeded in 2 stages of different reaction velocity. The 1st stage is considered as an inductive period. The rate constant and energy of activation of the 2nd stage reaction are (1 ± 0.1) × 102 l. mole-1 sec.-1 and 8.2 kcal./mole, resp. Reaction rate was independent of H2SO4 or vanadium (V) concentrations (at 5 × 10-2-2 × 10-3M NH4VO3) but depended on MnO4- and VO2+ concentrations Phosphate-containing compounds (inorganic or purine and pyrimidine derivatives of phosphoric acid) accelerated the oxidation and in their presence the reaction depended also on the P-compound concentration, hence was a 3rd-order reaction. This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3Recommanded Product: Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt).

Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. Solid acid catalysis, and the advantages often associated with their use, have been proved equally efficient for the synthesis of tetrahydrofurans or furans. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Recommanded Product: Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt

18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3

The Reopening Rate of the Fingers Domain Is a Determinant of Base Selectivity for RB69 DNA Polymerase was written by Lee, Harold R.; Wang, Mina; Konigsberg, William. And the article was included in Biochemistry on March 17,2009.Application In Synthesis of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt The following contents are mentioned in the article:

Two divalent metal ions are required for nucleotide incorporation by DNA polymerases. Here we use the bacteriophage RB69 DNA polymerase (RB69 pol) and the metal ion exchange-inert nucleotide analog rhodium(III) deoxythymidine triphosphate (Rh·dTTP) to investigate the requirements of metal binding to the A site and to the B site, independently. We show that while binding of a metal ion to the A site is required for the nucleotidyl transfer reaction to occur, this metal binding is insufficient to initiate the prechem. enzyme isomerization that has been observed with this polymerase. Moreover, we show that binding of a deoxynucleoside triphosphate (dNTP), in the absence of a catalytic metal ion, is sufficient to induce this conformational change. In this report, we also present several lines of evidence (from pulse-chase, rapid chem. quench-flow, and stopped-flow fluorescence experiments) for the reverse rate of the enzyme isomerization, closed to open, of a DNA polymerase complex. The implications of these data for the fidelity of DNA polymerization by RB69 pol are discussed. This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3Application In Synthesis of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt).

Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Application In Synthesis of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt

18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3

Exploring the Binding Ability of Phenanthroline-Based Polyammonium Receptors for Anions: Hints for Design of Selective Chemosensors for Nucleotides was written by Bazzicalupi, Carla; Bencini, Andrea; Biagini, Silvia; Faggi, Enrico; Meini, Stefano; Giorgi, Claudia; Spepi, Alessio; Valtancoli, Barbara. And the article was included in Journal of Organic Chemistry on October 2,2009.Product Details of 18423-43-3 The following contents are mentioned in the article:

The synthesis of receptor 2,6,10,14,18-pentaaza[20]-21,34-phenanthrolinophane (L1), containing a pentaamine chain linking the 2,9 positions of a phenanthroline unit, is reported. The protonation features of L1 and of receptor 2,6,10,14,18,22-hexaaza[23]-24,37-phenanthrolinophane (L2) have been studied by potentiometric, 1H NMR, and spectrofluorimetric measurements; this study points out that the fluorescent emission of both receptors depends on the protonation state of the polyamine chain. In fact, the receptors are emissive only at neutral or acidic pH values, where all the aliphatic amine groups are protonated. Potentiometric titrations show that L2 is able to bind selectively ATP over TTP, CTP, and GTP. This selectivity is lost in the case of L1. 1H and 31P NMR measurements and mol. mechanics calculations show that the phosphate chains of nucleotides give strong electrostatic and hydrogen-bonding interactions with the ammonium groups of the protonated receptors, while the nucleobases interact either via π-stacking with phenanthroline or via hydrogen bonding with the ammonium groups. Of note, MM calculations suggest that all nucleotides interact in an inclusive fashion. In fact, in all adducts the phosphate chain is enclosed within the receptor cavities. This structural feature is confirmed by the crystal structure of the [(H6L2)2(TTP)2(H2O)2]4+ adduct. Fluorescence emission measurements at different pH values show that L2 is also able to ratiometrically sense ATP in a narrow pH range, thanks to emission quenching due to a photoinduced electron transfer (PET) process from an amine group of the receptor to the excited phenanthroline. This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3Product Details of 18423-43-3).

Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). Commercial tetrahydrofuran contains substantial water that must be removed for sensitive operations, e.g. those involving organometallic compounds. Although tetrahydrofuran is traditionally dried by distillation from an aggressive desiccant, molecular sieves are superior.Product Details of 18423-43-3

18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3

A complementary pair of rapid molecular screening assays for RecA activities was written by Lee, Andrew M.; Wigle, Tim J.; Singleton, Scott F.. And the article was included in Analytical Biochemistry on August 15,2007.Application of 18423-43-3 The following contents are mentioned in the article:

The bacterial RecA protein has been implicated in the evolution of antibiotic resistance in pathogens, which is an escalating problem worldwide. The discovery of small mols. that can selectively modulate RecA’s activities can be exploited to tease apart its roles in the de novo development and transmission of antibiotic resistance genes. Toward the goal of discovering small-mol. ligands that can prevent either the assembly of an active RecA-DNA filament or its subsequent ATP-dependent motor activities, we report the design and initial validation of a pair of rapid and robust screening assays suitable for the identification of inhibitors of RecA activities. One assay is based on established methods for monitoring ATPase enzyme activity and the second is a novel assay for RecA-DNA filament assembly using fluorescence polarization. Taken together, the assay results reveal complementary sets of agents that can either suppress selectively only the ATP-driven motor activities of the RecA-DNA filament or prevent assembly of active RecA-DNA filaments altogether. The screening assays can be readily configured for use in future automated high-throughput screening projects to discover potent inhibitors that may be developed into novel adjuvants for antibiotic chemotherapy that moderate the development and transmission of antibiotic resistance genes and increase the antibiotic therapeutic index. This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3Application of 18423-43-3).

Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. Solid acid catalysis, and the advantages often associated with their use, have been proved equally efficient for the synthesis of tetrahydrofurans or furans. THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.Application of 18423-43-3

18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3

Pharmacological features of the coronary, renal, mesenteric, and femoral vascular beds of rats revealed by intra-arterial administration of drugs was written by Sakai, Kazushige; Akima, Michitaka; Adachi, Jiro. And the article was included in Journal of Cardiovascular Pharmacology on August 31,1980.Computed Properties of C10H14N2Na3O14P3 The following contents are mentioned in the article:

The effects of drugs on circulation in the isolated, blood-perfused heart, kidney, small intestine, and hindlimb of rats were compared to previously reported results in dogs. Single intraarterial injections were made into the perfusion system of the coronary, renal, mesenteric, or femoral vascular bed. The most striking differences between rats and dogs were observed in responses to 1-nicotine d-bitartrate [65-31-6], dipyridamole [58-32-2], 5-hydroxytryptamine creatinine sulfate (5-HT) [971-74-4], lobeline-HCl [134-63-4], tetraethylammonium chloride (TEA) [56-34-8], and procaine-HCl [51-05-8]. Nicotine constricted all vascular beds except the coronary bed in dogs, but dilated all 4 beds in rats. Dipyridamole dilated all beds in rats, but constricted the renal vascular bed of dogs. 5-HT constricted all vascular beds of rats, but dilated the coronary vasculature of dogs. Lobeline, TEA, and procaine constricted only the renal vasculature of dogs, but constricted all vascular beds of rats. This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3Computed Properties of C10H14N2Na3O14P3).

Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF), or oxolane, is mainly used as a precursor to polymers. Being polar and having a wide liquid range, THF is a versatile solvent. Commercial tetrahydrofuran contains substantial water that must be removed for sensitive operations, e.g. those involving organometallic compounds. Although tetrahydrofuran is traditionally dried by distillation from an aggressive desiccant, molecular sieves are superior.Computed Properties of C10H14N2Na3O14P3

18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3

Exploring metabolic adaptation of Streptococcus pneumoniae to antibiotics was written by Leonard, Anne; Moehlis, Kevin; Schlueter, Rabea; Taylor, Edward; Lalk, Michael; Methling, Karen. And the article was included in Journal of Antibiotics on July 31,2020.SDS of cas: 18423-43-3 The following contents are mentioned in the article:

Abstract: The Gram-pos. bacterium Streptococcus pneumoniae is one of the common causes of community acquired pneumonia, meningitis, and otitis media. Analyzing the metabolic adaptation toward environmental stress conditions improves our understanding of its pathophysiol. and its dependency on host-derived nutrients. In this study, extra- and intracellular metabolic profiles were evaluated to investigate the impact of antimicrobial compounds targeting different pathways of the metabolome of S. pneumoniae TIGR4Δcps. For the metabolomics approach, we analyzed the complex variety of metabolites by using 1H NMR, HPLC-MS, and GC-MS as different anal. techniques. Through this combination, we detected nearly 120 metabolites. For each antimicrobial compound, individual metabolic effects were detected that often comprised global biosynthetic pathways. Cefotaxime altered amino acids metabolism and carbon metabolism The purine and pyrimidine metabolic pathways were mostly affected by moxifloxacin treatment. The combination of cefotaxime and azithromycin intensified the stress response compared with the use of the single antibiotic. Teixobactin-Arg10 resulted in global changes of pneumococcal metabolism To meet the growing requirements for new antibiotics, our metabolomics approach has shown to be a promising complement to other OMICs investigations allowing insights into the mode of action of novel antimicrobial compounds This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3SDS of cas: 18423-43-3).

Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is a stable compound with relatively low boiling point and excellent solvency. Tetrahydrofuran (THF) is primarily used as a precursor to polymers including for surface coating, adhesives, and printing inks.SDS of cas: 18423-43-3

18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3

Separation of nucleotides by hydrophilic interaction chromatography using the FRULIC-N column was written by Padivitage, Nilusha L. T.; Dissanayake, Milan K.; Armstrong, Daniel W.. And the article was included in Analytical and Bioanalytical Chemistry on November 30,2013.Safety of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt The following contents are mentioned in the article:

A stationary phase composed of silica-bonded cyclofructan 6 (FRULIC-N) was evaluated for the separation of four cyclic nucleotides, six nucleoside monophosphates, four nucleoside diphosphates, and five nucleoside triphosphates via hydrophilic interaction chromatog. (HILIC) in both isocratic and gradient conditions. The gradient conditions gave significantly better separations by narrowing peak widths. Sixteen out of nineteen nucleotides were baseline separated on the FRULIC-N column in one run. Unlike other known HILIC stationary phases, there can be dual-retention mechanisms unique to this media. Traditional hydrogen bonding/dipolar interactions can be supplemented by dynamic ion interaction effects for anionic analytes. This occurs because the FRULIC-N stationary phase is able to bind certain buffer cations. The extent of the ion interaction is tunable, in comparison to stationary phases with embedded charged groups, where the inherent ionic properties are fixed. The best mobile phase conditions were determined by varying the organic modifier (acetonitrile) content, as well as salt type/concentration and electrolyte pH. The thermodn. characteristic of the FRULIC-N column was investigated by evaluating the column temperature effect on retention and utilizing van’t Hoff plots. This study shows that there is a greater entropic contribution for the retention of nucleotide di and triphosphates, whereas there is a greater enthalphic contribution for the cyclic nucleotides with the FRULIC-N column. This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3Safety of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt).

Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF), or oxolane, is mainly used as a precursor to polymers. Being polar and having a wide liquid range, THF is a versatile solvent. Tetrahydrofuran can also be produced, or synthesised, via catalytic hydrogenation of furan. This process involves converting certain sugars into THF by digesting to furfural. An alternative to this method is the catalytic hydrogenation of furan with a nickel catalyst.Safety of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt

18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3