Anti-birnavirus activity of methisoprinol – in vitro study with infectious pancreatic necrosis virus (IPNV) was written by Siwicki, A. K.;Morand, M.;Pozet, F.;Kazun, B.. And the article was included in Acta Veterinaria Brno in 2002.Application of 36703-88-5 This article mentions the following:
This study was conducted to evaluate the influence of methisoprinol, synthetic anti-viral product, on the IPNV replication in vitro by measuring viral RNA synthesis. The monolayers of RTG-2 cells in tissue culture plates (Multiwell, 24 wells, Becton Dickinson, USA) were cultivated with different concentrations of methisoprinol (Polfa, Poland) 0, 100, 200, 400, 500 and 1000 μg/mL of medium and were followed by infection with 100 μl of IPN virus suspension containing 107 TCID50/mL, in triplicate. At 24, 48 and 72 h after infection, the IPN virus-infected and methisoprinol-treated RTG-2 cell cultures were submitted to one hour starvation and after two hours incubation with 10 μCi/mL of [3H]-uridine. Culture homogenates of each isolate were incubated with phenol/chloroform to extract RNA and followed by slab PAGE for 2 h. The gel strips were dissolved and the counts per min (cpm) evaluated in a scintillation counter. The replicative cycle of IPN virus in RTG-2 cell culture was rapid. In control group (only infected by IPNV), the incorporation of [3H]-uridine was 45,000±1500 cpm at 24 h, 186,000±2450 cpm at 48 h and 554,500±4 550 cpm at 72 h. The percent of inhibition of IPN viral RNA labeling under methisoprinol application ranged from 5% at 24 h to 85% at 72 h depending on concentration of tested product and time when cultures were harvested. The highest percent of inhibition at 72 h after infection was observed at the dose 1000 μg/mL. The results of these exptl. studies show the inhibition of incorporation (cpm) of [3H]-uridine into IPN viral RNA in cell cultures exposed with methisoprinol at various concentrations In the experiment, the researchers used many compounds, for example, 4-Acetamidobenzoic acid,9-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3H-purin-6(9H)-one,1-(Dimethylamino)propan-2-ol (3:1:3) (cas: 36703-88-5Application of 36703-88-5).
4-Acetamidobenzoic acid,9-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3H-purin-6(9H)-one,1-(Dimethylamino)propan-2-ol (3:1:3) (cas: 36703-88-5) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.Application of 36703-88-5
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem