Thyroid purine nucleoside phosphorylase. II. Kinetic model by alternate substrate and inhibition studies was written by Carlson, Jeffrey D.; Fischer, Allan G.. And the article was included in Biochimica et Biophysica Acta, Enzymology in 1979.Related Products of 13146-72-0 The following contents are mentioned in the article:
Nucleoside analog inhibition studies were conducted on thyroidal purine nucleoside phosphorylase which catalyzed an ordered bi-bi-type mechanism in which the 1st substrate is inorganic phosphate and the last product is ribose 1-phosphate. Heterocyclic- and carbohydrate-modified nucleoside inhibitors demonstrated mixed-type inhibition, suggesting that such analogs show an affinity (Ki) for the free enzyme. A kinetic model is proposed which supports the observed inhibition patterns. These studies, together with alternate substrate studies, indicated that nucleoside binding requires a functional group capable of H bonding at the 6 position of the purine ring and that the orientation of the bound substrate may be syn. Proper geometry of the phosphate is dependent on the 3′ substituent being oriented below the furanose ring. The 5′-OH group is required for substrate activity. The proposed rate-limiting step of the phosphorylase mechanism is the enzymic protonation of the N-7 position of the nucleoside. This study involved multiple reactions and reactants, such as 9-((2R,3R,5S)-3-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-9H-purin-6-ol (cas: 13146-72-0Related Products of 13146-72-0).
9-((2R,3R,5S)-3-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-9H-purin-6-ol (cas: 13146-72-0) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is a stable compound with relatively low boiling point and excellent solvency. Oxidations have also proved to be valuable and efficient approaches to chiral tetrahydrofuran derivatives.Related Products of 13146-72-0
13146-72-0;9-((2R,3R,5S)-3-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-9H-purin-6-ol;The future of 13146-72-0;New trend of C10H12N4O4 ;function of 13146-72-0