Inhibition of protein N-glycosylation by 2-deoxy-2-fluoro-D-galactose was written by Gross, Volker; Hull, William E.; Berger, Ulrike; Andus, Tilo; Kreisel, Wolfgang; Gerok, Wolfgang; Keppler, Dietrich. And the article was included in Biochemical Journal on August 1,1992.Application of 67341-43-9 The following contents are mentioned in the article:
The effects of 2-deoxy-2-fluoro-D-galactose (dGalF) on N- and O-glycosylation of proteins was studied in rat hepatocyte primary cultures and in human monocytes. In hepatocytes, dGalF at concentrations of ≥1 mM completely inhibited N-glycosylation of α1-antitrypsin and α1-acid glycoprotein, whereas 4 mM 2-deoxy-D-galactose (dGal) only slightly impaired N-glycosylation. In monocytes, 1 mM or 4 mM dGalF blocked N-glycosylation of α1-antitrypsin and interleukin-6, whereas O-glycosylation of interleukin-5 remained unaffected. In monocytes, dGal had no effect on protein N-glycosylation. Addition of uridine effectively prevented the UTP deficiency induced by dGalF, but had no effect on the inhibition of protein N-glycosylation by dGalF. Using 19F-NMR spectroscopy, 2-deoxy-2-fluoro-D-galactose 1-phosphate (dGalF-1P), UDP-dGalF, and UDP-dGlcF could be identified as the major metabolites of dGalF in hepatocytes as well as in monocytes. In conclusion, compared with dGal, dGalF is a more efficient inhibitor of protein N-glycosylation. The effect is not caused by the depletion of UTP induced by dGalF, but rather by metabolites of dGalF. The dGalF is metabolized not only in hepatocytes but also in peripheral blood monocytes, which can be used for ex vivo studies of disturbances in D-galactose metabolism This study involved multiple reactions and reactants, such as Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester (cas: 67341-43-9Application of 67341-43-9).
Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester (cas: 67341-43-9) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Application of 67341-43-9
67341-43-9;Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester;The future of 67341-43-9;New trend of C15H23FN2O16P2 ;function of 67341-43-9