Feliu, Catherine; Peyret, Helene; Vautier, Damien; Djerada, Zoubir published the artcile< Simultaneous quantification of 8 nucleotides and adenosine in cells and their medium using UHPLC-HRMS>, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate, the main research area is nucleotide adenosine UHPLC HRMS; Adenosine; Extracellular; High-resolution mass spectrometry; Intracellular; Nucleotides; Quantification.
Purinergic signaling is involved in physiol. processes, particularly during ischemia-reperfusion injuries for which it has a protective effect. The purpose of this work was to develop a method for simultaneous quantification of eight nucleotides and adenosine in biol. matrixes by liquid chromatog. coupled with high-resolution mass spectrometry. A method was developed that was sufficiently robust to quantify the targeted analytes in 20 min with good sensitivity. Anal. of extracellular media from cultured endothelial cells detected the release of nucleotides and adenosine during 2 h of hypoxia. The quantification of cylic adenosine monophosphate (cAMP) allowed to establish a dose-response curve after receptor stimulation. Therefore, the authors′ method allows the authors to study the involvement of nucleotides in various processes in both the intracellular and extracellular compartment.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences published new progress about Cardiomyocyte. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Recommanded Product: ((2R,3S,4R,5R)-5-(2,4-Dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate.
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem