Regulation of alternative polyadenylation by the C2H2-zinc-finger protein Sp1 was written by Song, Jingwen;Nabeel-Shah, Syed;Pu, Shuye;Lee, Hyunmin;Braunschweig, Ulrich;Ni, Zuyao;Ahmed, Nujhat;Marcon, Edyta;Zhong, Guoqing;Ray, Debashish;Ha, Kevin C. H.;Guo, Xinghua;Zhang, Zhaolei;Hughes, Timothy R.;Blencowe, Benjamin J.;Greenblatt, Jack F.. And the article was included in Molecular Cell in 2022.HPLC of Formula: 118-00-3 This article mentions the following:
Alternative polyadenylation (APA) enhances gene regulatory potential by increasing the diversity of mRNA transcripts. 3′ UTR shortening through APA correlates with enhanced cellular proliferation and is a widespread phenomenon in tumor cells. Here, we show that the ubiquitously expressed transcription factor Sp1 binds RNA in vivo and is a common repressor of distal poly(A) site usage. RNA sequencing identified 2,344 genes (36% of the total mapped mRNA transcripts) with lengthened 3′ UTRs upon Sp1 depletion. Sp1 preferentially binds the 3′ UTRs of such lengthened transcripts and inhibits cleavage at distal sites by interacting with the subunits of the core cleavage and polyadenylation (CPA) machinery. The 3′ UTR lengths of Sp1 target genes in breast cancer patient RNA-seq data correlate with Sp1 expression levels, implicating Sp1-mediated APA regulation in modulating tumorigenic properties. Taken together, our findings provide insights into the mechanism for dynamic APA regulation by unraveling a previously unknown function of the DNA-binding transcription factor Sp1. In the experiment, the researchers used many compounds, for example, 2-Amino-9-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3H-purin-6(9H)-one (cas: 118-00-3HPLC of Formula: 118-00-3).
2-Amino-9-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3H-purin-6(9H)-one (cas: 118-00-3) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). THF (Tetrahydrofuran) is also used as a starting material for the synthesis of poly(tetramethylene ether) glycol (PTMG), etc.HPLC of Formula: 118-00-3
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem