Cytokinin oxidase/dehydrogenase OsCKX11 coordinates source and sink relationship in rice by simultaneous regulation of leaf senescence and grain number was written by Zhang, Wei;Peng, Kaixuan;Cui, Fubin;Wang, Dongling;Zhao, Jiangzhe;Zhang, Yanjun;Yu, Ningning;Wang, Yuyang;Zeng, Dali;Wang, Yonghong;Cheng, Zhukuan;Zhang, Kewei. And the article was included in Plant Biotechnology Journal in 2021.Quality Control of N6-(3-Methyl-2-butenyl)adenosine This article mentions the following:
The flag leaf and grain belong to the source and sink, resp., of cereals, and both have a bearing on final yield. Premature leaf senescence significantly reduces the photosynthetic rate and severely lowers crop yield. Cytokinins play important roles in leaf senescence and determine grain number Here, we characterized the roles of the rice (Oryza sativa L.) cytokinin oxidase/dehydrogenase OsCKX11 in delaying leaf senescence, increasing grain number, and coordinately regulating source and sink. OsCKX11 was predominantly expressed in the roots, leaves, and panicles and was strongly induced by abscisic acid and leaf senescence. Recombinant OsCKX11 protein catalyzed the degradation of various types of cytokinins but showed preference for trans-zeatin and cis-zeatin. Cytokinin levels were significantly increased in the flag leaves of osckx11 mutant compared to those of the wild type (WT). In the osckx11 mutant, the ABA-biosynthesizing genes were down-regulated and the ABA-degrading genes were up-regulated, thereby reducing the ABA levels relative to the WT. Thus, OsCKX11 functions antagonistically between cytokinins and ABA in leaf senescence. Moreover, osckx11 presented with significantly increased branch, tiller, and grain number compared with the WT. Collectively, our findings reveal that OsCKX11 simultaneously regulates photosynthesis and grain number, which may provide new insights into leaf senescence and crop mol. breeding. In the experiment, the researchers used many compounds, for example, N6-(3-Methyl-2-butenyl)adenosine (cas: 7724-76-7Quality Control of N6-(3-Methyl-2-butenyl)adenosine).
N6-(3-Methyl-2-butenyl)adenosine (cas: 7724-76-7) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Quality Control of N6-(3-Methyl-2-butenyl)adenosine
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem