Fluorescent Probes for Ecto-5′-nucleotidase (CD73) was written by Schmies, Constanze C.;Rolshoven, Georg;Idris, Riham M.;Losenkova, Karolina;Renn, Christian;Schaekel, Laura;Al-Hroub, Haneen;Wang, Yulu;Garofano, Francesca;Schmidt-Wolf, Ingo G. H.;Zimmermann, Herbert;Yegutkin, Gennady G.;Mueller, Christa E.. And the article was included in ACS Medicinal Chemistry Letters in 2020.Reference of 3056-18-6 This article mentions the following:
Ecto-5′-nucleotidase (CD73) catalyzes the hydrolysis of AMP to anti-inflammatory, immunosuppressive adenosine. It is expressed on vascular endothelial, epithelial, and also numerous cancer cells where it strongly contributes to an immunosuppressive microenvironment. In the present study we designed and synthesized fluorescent-labeled CD73 inhibitors with low nanomolar affinity and high selectivity based on N6-benzyl-α,β-methylene-ADP (PSB-12379) as a lead structure. Fluorescein was attached to the benzyl residue via different linkers resulting in PSB-19416 (14b, Ki 12.6 nM) and PSB-18332 (14a, Ki 2.98 nM) as fluorescent high-affinity probes for CD73. These compounds are anticipated to become useful tools for biol. studies, drug screening, and diagnostic applications. In the experiment, the researchers used many compounds, for example, (2R,3R,4R,5R)-2-(Acetoxymethyl)-5-(2,6-dichloro-9H-purin-9-yl)tetrahydrofuran-3,4-diyl diacetate (cas: 3056-18-6Reference of 3056-18-6).
(2R,3R,4R,5R)-2-(Acetoxymethyl)-5-(2,6-dichloro-9H-purin-9-yl)tetrahydrofuran-3,4-diyl diacetate (cas: 3056-18-6) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is water-miscible and has a low viscosity making it a highly versatile solvent used in a variety of industries. Tetrahydrofuran reaction with hydrogen sulfide: In the presence of a solid acid catalyst, tetrahydrofuran reacts with hydrogen sulfide to give tetrahydrothiophene.Reference of 3056-18-6
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem