Antibodies raised against the adenosine receptor agonist, 5′-N-ethylcarboxamidoadenosine (NECA) was written by Nakata, Hiroyasu. And the article was included in Journal of Biochemistry in 1993.Formula: C10H13N5O2 This article mentions the following:
Antisera against the nonselective adenosine receptor agonist 5′-N-ethylcarboxamidoadenosine (NECA) were raised by immunizing rabbits with NECA-coupled bovine serum albumin. The antisera which bind [3H]NECA with high affinity were purified by affinity chromatog. using NECA-coupled Sepharose as the affinity gel without significant changes in [3H]NECA-binding properties. The order of the affinity for various adenosine receptor ligands of the purified or unpurified antisera was 5′-N-cyclopropylcarboxamidoadenosine ≥ NECA>2′,5′-dideoxyadenosine>2-chloroadenosine > theophylline > IBMX > (R)-N6-phenylisopropyladenosine = N6-cyclohexyladenosine. The specificity was similar to that of the nonreceptor NECA-binding sites, which had been found in various tissues such as human placetas and mouse P815 mastocytoma cells, rather than to that of adenosine receptors. In the experiment, the researchers used many compounds, for example, (2R,3S,5R)-5-(6-Amino-9H-purin-9-yl)-2-methyltetrahydrofuran-3-ol (cas: 6698-26-6Formula: C10H13N5O2).
(2R,3S,5R)-5-(6-Amino-9H-purin-9-yl)-2-methyltetrahydrofuran-3-ol (cas: 6698-26-6) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is water-miscible and has a low viscosity making it a highly versatile solvent used in a variety of industries. Oxidations have also proved to be valuable and efficient approaches to chiral tetrahydrofuran derivatives.Formula: C10H13N5O2
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem