Quantitative measurement of mRNA cap 0 and cap 1 structures by high-performance liquid chromatography was written by Kuo, Kenneth C.;Smith, Christine E.;Shi, Zhixian;Agris, Paul F.;Gehrke, Charles W.. And the article was included in Journal of Chromatography, Biomedical Applications in 1986.HPLC of Formula: 2140-71-8 This article mentions the following:
The use of reversed-phase HPLC is described as a rapid and efficient tool for the separation, identification, and quantitation of caps of mRNA. An ion-exchange enrichment procedure was also developed for the isolation of cap 0 and cap 1 structures from unfractionated RNAs. The recoveries of different caps ranged 83-99%, with a relative standard deviation range of 1.3-4.4%. In this method, caps were released from com. obtained rabbit globin mRNA by nuclease P1 digestion. The products of digestion were treated with alk. phosphatase and separated on an octadecylsilyl column using stepwise or gradient elution. Cap structures and any internal modified nucleosides were identified by their retention times and UV spectra relative to reference compounds The amount of each cap 0 or cap 1 structure was determined by its UV absorbance relative to a known quality of reference compound This method allows the quantitation of ≥0.2 nmol of cap 0 and cap 1 structures. Total UV spectra can be obtained for ≥0.5 nmol of cap. This methodol. permits investigations on viral and eukaryotic mRNA cap biosynthesis and turnover during viral transformation, differentiation, cap synthesis in the cell cycle, etc. In the experiment, the researchers used many compounds, for example, 2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8HPLC of Formula: 2140-71-8).
2-Amino-9-((2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxytetrahydrofuran-2-yl)-1H-purin-6(9H)-one (cas: 2140-71-8) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). THF can also be synthesized by catalytic hydrogenation of furan. This allows certain sugars to be converted to THF via acid-catalyzed digestion to furfural and decarbonylation to furan, although this method is not widely practiced. THF is thus derivable from renewable resources.HPLC of Formula: 2140-71-8
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem