Metabolic significance of bisphenol A-induced oxidative stress in rat urine measured by liquid chromatography-mass spectrometry was written by Cho, Sung-Hee;Choi, Man Ho;Kwon, Oh Seung;Lee, Won-Yong;Chung, Bong Chul. And the article was included in Journal of Applied Toxicology in 2009.Formula: C10H14N2O6 This article mentions the following:
Modified nucleosides are formed by DNA repair as a result of oxidative DNA damage and post-transcriptionally modified tRNA in cells. In the present study, the profiling of 14 nucleosides was investigated in rat urine to evaluate bisphenol A (BPA)-induced oxidative stress after i.p. injecting rats with 0, 10, or 50 mg kg-1 per day of BPA for 4 consecutive days. The urinary concentrations of individual nucleosides were measured by liquid chromatog.-tandem mass spectrometry combined with column switching online extraction Increased levels of 5-hydroxymethyl-2′-deoxyuridine (P < 0.01 on first, P < 0.005 on second, P < 0.001 on third, and P < 0.01 on fourth day) and 8-hydroxy-2′-deoxyguanosine (P < 0.005 on second, P < 0.001 on third, and P < 0.001 on fourth day) were found. Also, the patterns of urinary nucleosides in 3 dosage groups (control, BPA1, BPA2) were significantly different. Statistical significance was observed between BPA1 (5-hydroxymethyl-2′-deoxyuridine, P < 0.05; 8-hydroxy-2′-deoxyguanosine, P < 0.005) and BPA2 (5-hydroxymethyl-2′-deoxyurindine, P < 0.005; 8-hydroxy-2′-deoxyguanosine, P < 0.001) during the treatment period. Supervised anal. with partial least-squares-discrimination anal. led to discrimination between the 3 dosage groups. Quant. alterations showed the metabolic trajectories responsible for physiol. responses. The described methods could be used to evaluate and monitor BPA-induced oxidatives stress early after exposure. In the experiment, the researchers used many compounds, for example, 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4Formula: C10H14N2O6).
1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-methylpyrimidine-2,4(1H,3H)-dione (cas: 2140-69-4) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is water-miscible and has a low viscosity making it a highly versatile solvent used in a variety of industries. Commercial tetrahydrofuran contains substantial water that must be removed for sensitive operations, e.g. those involving organometallic compounds. Although tetrahydrofuran is traditionally dried by distillation from an aggressive desiccant, molecular sieves are superior.Formula: C10H14N2O6
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem