Improvement of 1-Kestose-producing enzyme was written by Fujii, Tadashi;Tochio, Takumi. And the article was included in Oyo Toshitsu Kagaku in 2021.Related Products of 470-69-9 This article mentions the following:
Fructooligosaccharide (FOS) is a polymer in which β-2, 1-linked fructose units are transferred to sucrose and is widely used as a prebiotic. In particular, it has been reported that kestose, which is a trisaccharide FOS, has a particularly high prebiotic function, such as the selective growth of useful intestinal bacteria such as fatty acid-producing bacteria. In order to efficiently produce kestose, the author attempted to obtain an enzyme that specifically produces kestose by suppressing the byproduct of kestose, which is the FOS of tetrasaccharides. As a result of screening using the Escherichia coli surface presentation method, BiBftA H395R / F473Y, a variant of the enzyme derived from Beijerinckia Indica, specifically accumulated kestose. In particular, H395R is an important mutation, and it is considered that it suppresses the formation of long chains longer than kestose and also suppresses the degradation of sucrose without transglycosylation. As a result of confirming the time course of the reaction, about 44% of sucrose was converted to kestose by BiBftA H395R / H473Y, and at this time, the byproduct of sucrose was suppressed to about 1%. This improved enzyme was thought to contribute to the dramatic reduction in production cost and improvement in production efficiency of kestose crystals. In the experiment, the researchers used many compounds, for example, (2R,3R,4S,5S,6R)-2-(((2S,3S,4S,5R)-2-((((2R,3S,4S,5R)-3,4-Dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl)oxy)methyl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 470-69-9Related Products of 470-69-9).
(2R,3R,4S,5S,6R)-2-(((2S,3S,4S,5R)-2-((((2R,3S,4S,5R)-3,4-Dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl)oxy)methyl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 470-69-9) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is water-miscible and has a low viscosity making it a highly versatile solvent used in a variety of industries. Tetrahydrofuran (THF) is primarily used as a precursor to polymers including for surface coating, adhesives, and printing inks.Related Products of 470-69-9
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem