LC-MS/MS method validation for the quantitation of 1-kestose in wheat flour was written by Fruehwirth, Sarah;Call, Lisa;Maier, Fabiola Abigail;Hebenstreit, Vanessa;D′Amico, Stefano;Pignitter, Marc. And the article was included in Journal of Food Composition and Analysis in 2021.Application In Synthesis of (2R,3R,4S,5S,6R)-2-(((2S,3S,4S,5R)-2-((((2R,3S,4S,5R)-3,4-Dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl)oxy)methyl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol This article mentions the following:
Fructan anal. is challenging due to several mono-, di-, and oligosaccharides as well as starch and proteins present in wheat grains which interfere with the anal. So far, a sensitive and fully validated LC-MS/MS method is missing although even small amounts of short-chain fructans such as 1-kestose are suggested to elicit gastrointestinal symptoms in patients suffering from irritable bowel syndrome. Therefore, the aim of this study was to validate a LC-MS/MS method for the detection of 1-kestose in a complex wheat matrix according to international guidelines. The chromatog. separation was performed on a C18 column coupled with a triple quadrupole MS in MRM mode using the transition m/z 503 → m/z 119 for the quantitation of 1kestose. Accuracy of 1-kestose quantitation in an aqueous solution was 105.5%. However, the poor recovery of 64.1% in a complex wheat matrix demonstrated the great impact of proteins and other constituents present in wheat. Nevertheless, the proposed method allows precise quantification of 1-kestose amounts ≥0.1 ppm. The robustness of the method was confirmed by the results being within 6.03% of the target value. The validated method was successfully applied for the measurement of 10 different wheat samples with 1-kestose amounts between 7.0 and 50.2 ppm. In the experiment, the researchers used many compounds, for example, (2R,3R,4S,5S,6R)-2-(((2S,3S,4S,5R)-2-((((2R,3S,4S,5R)-3,4-Dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl)oxy)methyl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 470-69-9Application In Synthesis of (2R,3R,4S,5S,6R)-2-(((2S,3S,4S,5R)-2-((((2R,3S,4S,5R)-3,4-Dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl)oxy)methyl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol).
(2R,3R,4S,5S,6R)-2-(((2S,3S,4S,5R)-2-((((2R,3S,4S,5R)-3,4-Dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl)oxy)methyl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 470-69-9) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Application In Synthesis of (2R,3R,4S,5S,6R)-2-(((2S,3S,4S,5R)-2-((((2R,3S,4S,5R)-3,4-Dihydroxy-2,5-bis(hydroxymethyl)tetrahydrofuran-2-yl)oxy)methyl)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem