Effect of 5-iodotubercidin on proliferation and apoptosis in human colon cancer HT-29 cells and demethylation of deleted in liver cancer-1 gene was written by Zhang, Lingmin;Wang, Yaxu;Xie, Kai;Zeng, Linghai;Li, Quan. And the article was included in Di-San Junyi Daxue Xuebao in 2015.Computed Properties of C11H13IN4O4 The following contents are mentioned in the article:
Objective: To explore the effect of 5-iodotubercidin (ITU) on the proliferation and apoptosis in human colon cancer HT-29 cells and on the expression of deleted in liver cancer-1 (DLC-1) gene. Methods: HT-29 cells were treated with different concentrations of ITU (0.1, 0.5, 1, 2, 4, 6, 8, and 10 μmol/L) for 48 h, and the cell viability was studied by MTT assay. HT-29 cells were co-cultured with different concentrations of ITU (0, 2, 4, and 6 μmol/L) for 48 h, and the apoptosis rate was detected by flow cytometry. Meanwhile, the methylation levels of CpG islands at promoter region of DLC-1 gene were determined by bisulfite sequencing PCR (BSP). The DLC-1 mRNA expression levels were detected by reverse transcription-polymerase chain reaction (RT-PCR) and the DLC-1 protein expression levels were detected by Western blotting. Results: ITU distinctly inhibited the proliferation of HT-29 cells in a concentration-dependent manner in a certain range, and the 6 μmol/L group had the greatest effect, with IC50 of 5.92 ± 0.62 μmol/L. The apoptotic rates of HT-29 cells treated with ITU (0, 2, 4, and 6 μmol/L) were (9.56 ± 2.21)%, (22.18 ± 3.16)%, (34.23 ± 3.16)%, and (40.5 ± 3.16)%, resp., which were increased in a concentration-dependent manner. The methylation rates of CpG islands at the promoter region of DLC-1 gene were 91.67%, 75.00%, 61.67%, and 43.33%, resp., after treatment with ITU (0, 2, 4, and 6 μmol/L). The results showed that ITU could decrease the methylation of DLC-1 gene promoter region. Little of DLC-1 mRNA and protein expression was detected in HT-29 cells, and ITU treatment increased the expression in a concentration-dependent manner. Conclusion: ITU could inhibit the proliferation of HT-29 cells and induce apoptosis through decreasing the methylation level of DLC-1 gene promoter region and increasing the DLC-1 gene expression. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R)-2-(4-Amino-5-iodo-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol (cas: 24386-93-4Computed Properties of C11H13IN4O4).
(2R,3R,4S,5R)-2-(4-Amino-5-iodo-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol (cas: 24386-93-4) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF) is a Lewis base that bonds to a variety of Lewis acids such as I2, phenols, triethylaluminum and bis(hexafluoroacetylacetonato)copper(II). Tetrahydrofuran reaction with hydrogen sulfide: In the presence of a solid acid catalyst, tetrahydrofuran reacts with hydrogen sulfide to give tetrahydrothiophene.Computed Properties of C11H13IN4O4
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem