The development and validation of a novel LC-MS/MS method for the simultaneous quantification of Molnupiravir and its metabolite ss-d-N4-hydroxycytidine in human plasma and saliva was written by Amara, Alieu;Penchala, Sujan Dilly;Else, Laura;Hale, Colin;FitzGerald, Richard;Walker, Lauren;Lyons, Rebecca;Fletcher, Tom;Khoo, Saye. And the article was included in Journal of Pharmaceutical and Biomedical Analysis in 2021.Category: tetrahydrofurans The following contents are mentioned in the article:
In light of the recent global pandemic, Molnupiravir (MPV) or EIDD-2801, developed for the treatment of patients with uncomplicated influenza, is now being trialed for the treatment of infections caused by highly pathogenic coronaviruses, including COVID-19. A sensitive LC-MS/MS method was developed and validated for the simultaneous quantification of MPV and its metabolite ss-d-N4-hydroxycytidine (NHC) in human plasma and saliva. The analytes were extracted from the matrixes by protein precipitation using acetonitrile. This was followed by drying and subsequently injecting the reconstituted solutions onto the column. Chromatog. separation was achieved using a polar Atlantis C18 column with gradient elution of 1 mM Ammonium acetate in water (pH 4.3) and 1 mM Ammonium acetate in acetonitrile. Analyte detection was conducted in neg. ionization mode using SRM. Anal. was performed using stable isotopically labeled (SIL) internal standards (IS). The m/z transitions were: MPV (328.1→126.0), NHC (258.0→125.9), and MPV-SIL (331.0→129.0), NHC-SIL (260.9→128.9). Validation was over a linear range of 2.5-5000 ng/mL for both plasma and saliva. Across 4 different concentrations, precision and accuracy (intra- and inter-day) were 15%; and recovery of both analytes from plasma and saliva was 95-100% and 65-86%, resp. Clin. pharmacokinetic studies are underway utilizing this method for determination of MPV and its metabolite in patients with COVID-19 infection. This study involved multiple reactions and reactants, such as ((2R,3S,4R,5R)-3,4-Dihydroxy-5-((Z)-4-(hydroxyimino)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl isobutyrate (cas: 2492423-29-5Category: tetrahydrofurans).
((2R,3S,4R,5R)-3,4-Dihydroxy-5-((Z)-4-(hydroxyimino)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl isobutyrate (cas: 2492423-29-5) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is a stable compound with relatively low boiling point and excellent solvency. Commercial tetrahydrofuran contains substantial water that must be removed for sensitive operations, e.g. those involving organometallic compounds. Although tetrahydrofuran is traditionally dried by distillation from an aggressive desiccant, molecular sieves are superior.Category: tetrahydrofurans
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem