Yang, Kun published the artcileChemical Protein Ubiquitylation with Preservation of the Native Cysteine Residues, Application In Synthesis of 57124-87-5, the publication is ChemBioChem (2016), 17(11), 995-998, database is CAplus and MEDLINE.
We report a cysteine-based ligation strategy for generating a monoubiquitylated protein while preserving the native cysteine residues on the acceptor protein. In monoubiquitylation of proliferating cell nuclear antigen (PCNA) this method circumvents the need to mutate the native cysteine residues on PCNA. The chem. ubiquitylated PCNA contains a noncleavable linkage of the same length as the native isopeptide linkage. It also retains the normal function of the native Ub-PCNA in stimulating the ATPase activity of replication factor C (RFC) and lesion bypass synthesis by Polη. This method may be adapted for chem. ubiquitylation of other proteins and for site-specific modification of a target protein at a specific site through sulfhydryl chem.
ChemBioChem published new progress about 57124-87-5. 57124-87-5 belongs to tetrahydrofurans, auxiliary class Tetrahydrofuran,Thiol, name is 2-Methyl-3-tetrahydrofuranthiol, and the molecular formula is C9H10F3NO2S, Application In Synthesis of 57124-87-5.
Referemce:
https://en.wikipedia.org/wiki/Tetrahydrofuran,
Tetrahydrofuran | (CH2)3CH2O – PubChem