The Reopening Rate of the Fingers Domain Is a Determinant of Base Selectivity for RB69 DNA Polymerase was written by Lee, Harold R.; Wang, Mina; Konigsberg, William. And the article was included in Biochemistry on March 17,2009.Application In Synthesis of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt The following contents are mentioned in the article:
Two divalent metal ions are required for nucleotide incorporation by DNA polymerases. Here we use the bacteriophage RB69 DNA polymerase (RB69 pol) and the metal ion exchange-inert nucleotide analog rhodium(III) deoxythymidine triphosphate (Rh·dTTP) to investigate the requirements of metal binding to the A site and to the B site, independently. We show that while binding of a metal ion to the A site is required for the nucleotidyl transfer reaction to occur, this metal binding is insufficient to initiate the prechem. enzyme isomerization that has been observed with this polymerase. Moreover, we show that binding of a deoxynucleoside triphosphate (dNTP), in the absence of a catalytic metal ion, is sufficient to induce this conformational change. In this report, we also present several lines of evidence (from pulse-chase, rapid chem. quench-flow, and stopped-flow fluorescence experiments) for the reverse rate of the enzyme isomerization, closed to open, of a DNA polymerase complex. The implications of these data for the fidelity of DNA polymerization by RB69 pol are discussed. This study involved multiple reactions and reactants, such as Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3Application In Synthesis of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt).
Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt (cas: 18423-43-3) belongs to tetrahydrofuran derivatives. Tetrahydrofurans and furans are important oxygen-containing heterocycles that often exhibit interesting properties for biological applications or applications in the cosmetic industry. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Application In Synthesis of Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt
18423-43-3;Thymidine 5′-(tetrahydrogen triphosphate) xsodium salt;The future of 18423-43-3;New trend of C10H14N2Na3O14P3;function of 18423-43-3