The Donor Subsite of Trehalose-6-phosphate Synthase: Binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 Å resolution was written by Gibson, Robert P.; Tarling, Chris A.; Roberts, Shirley; Withers, Stephen G.; Davies, Gideon J.. And the article was included in Journal of Biological Chemistry on January 16,2004.Name: Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester The following contents are mentioned in the article:
Trehalose is an unusual non-reducing disaccharide that plays a variety of biol. roles, from food storage to cellular protection from environmental stresses such as desiccation, pressure, heat-shock, extreme cold, and oxygen radicals. It is also an integral component of the cell-wall glycolipids of mycobacteria. The primary enzymic route to trehalose first involves the transfer of glucose from a UDP-glucose donor to glucose-6-phosphate to form α,α-1,1 trehalose-6-phosphate. This reaction, in which the configurations of two glycosidic bonds are set simultaneously, is catalyzed by the glycosyltransferase trehalose-6-phosphate synthase (OtsA), which acts with retention of the anomeric configuration of the UDP-sugar donor. The classification of activated sugar-dependent glycosyltransferases into approx. 70 distinct families based upon amino acid sequence similarities places OtsA in glycosyltransferase family 20 (see afmb.cnrs-mrs.fr/CAZY/). The recent 2.4 Å structure of Escherichia coli OtsA revealed a two-domain enzyme with catalysis occurring at the interface of the twin β/α/β domains. Here we present the 2.0 Å structures of the E. coli OtsA in complex with either UDP-Glc or the non-transferable analog UDP-2-deoxy-2-fluoroglucose. Both complexes unveil the donor subsite interactions, confirming a strong similarity to glycogen phosphorylases, and reveal substantial conformational differences to the previously reported complex with UDP and glucose 6-phosphate. Both the relative orientation of the two domains and substantial (up to 10 Å) movements of an N-terminal loop (residues 9-22) characterize the more open “”relaxed”” conformation of the binary UDP-sugar complexes reported here. This study involved multiple reactions and reactants, such as Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester (cas: 67341-43-9Name: Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester).
Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester (cas: 67341-43-9) belongs to tetrahydrofuran derivatives. THF (Tetrahydrofuran) is a stable compound with relatively low boiling point and excellent solvency. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Name: Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester
67341-43-9;Uridine 5′-(trihydrogen diphosphate) P’-(2-deoxy-2-fluoro-α-D-glucopyranosyl) ester;The future of 67341-43-9;New trend of C15H23FN2O16P2 ;function of 67341-43-9