Methods for preparing and using tagged nucleotide compositions for nanopore based sequencing was written by Fuller, Carl W.; Kumar, Shiv; Ju, Jingyue; Davis, Randall; Chen, Roger. And the patent was published on October 1,2015.Formula: C10H12N4O4 The following contents are mentioned in the patent:
This disclosure provides systems and methods for attaching nano pore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disclosed tagged nucleotides. Provided herein are nucleotides with attached tags and methods for attaching tags to nucleotides. The tags can be attached by chem. reactions, such as click chem. In an aspect, the present disclosure provides a tagged nucleotide, comprising a poly-phosphate moiety having a terminal phosphate and a tag covalently coupled to the terminal phosphate of the nucleotide by a triazole, a 1,2-diazine, a disulfide, a secondary amine, a hydrazone, a thio-acetamide, or a maleimide-thioadduct. This study involved multiple reactions and reactants, such as (2R,3S,4R,5R)-2-(Hydroxymethyl)-5-(9H-purin-9-yl)tetrahydrofuran-3,4-diol (cas: 550-33-4Formula: C10H12N4O4 ).
(2R,3S,4R,5R)-2-(Hydroxymethyl)-5-(9H-purin-9-yl)tetrahydrofuran-3,4-diol (cas: 550-33-4) belongs to tetrahydrofuran derivatives. Tetrahydrofuran (THF), or oxolane, is mainly used as a precursor to polymers. Being polar and having a wide liquid range, THF is a versatile solvent. It is more basic than diethyl ether and forms stronger complexes with Li+, Mg2+, and boranes. It is a popular solvent for hydroboration reactions and for organometallic compounds such as organolithium and Grignard reagents.Formula: C10H12N4O4
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem