Chandra, Anshuman; Gurjar, Vaishali; Qamar, Imteyaz; Singh, Nagendra published the artcile< Identification of potential inhibitors of SARS-COV-2 endoribonuclease (EndoU) from FDA approved drugs: a drug repurposing approach to find therapeutics for COVID-19>, Computed Properties of 58-97-9, the main research area is SARS COV2 endoribonuclease EndoU inhibitor drug repurposing; IC50; SARS-CoV-2; binding affinity; drug repurposing; endonuclease; molecular dynamic simulation.
SARS-CoV-2 is causative agent of COVID-19, which is responsible for severe social and economic disruption globally. Lack of vaccine or antiviral drug with clin. efficacy suggested that drug repurposing approach may provide a quick therapeutic solution to COVID-19. Nonstructural protein-15 (NSP15) encodes for an uridylate-specific endoribonuclease (EndoU) enzyme, essential for virus life cycle and an attractive target for drug development. We have performed in silico based virtual screening of FDA approved compounds targeting EndoU in search of COVID-19 drugs from com. available approved mols. Two drugs Glisoxepide and Idarubicin used for treatment for diabetes and leukemia, resp., were selected as stronger binder of EndoU. Both the drugs bound to the active site of the viral endonuclease by forming attractive intermol. interactions with catalytically essential amino acid residues, His235, His250, and Lys290. Mol. dynamics simulation studies showed stable conformation dynamics upon drugs binding to endoU. The binding free energies for Glisoxepide and Idarubicin were calculated to be -141±11 and -136±16kJ/mol, resp. The IC50 were predicted to be 9.2μM and 30μM for Glisoxepide and Idarubicin, resp. Comparative structural anal. showed the stronger binding of EndoU to Glisoxepide and Idarubicin than to uridine monophosphate (UMP). Surface area calculations showed buried are of 361.8 Å2 by Glisoxepide which is almost double of the area occupied by UMP suggesting stronger binding of the drug than the ribonucleotide. However, further studies on these drugs for evaluation of their clin. efficacy and dose formulations may be required, which may provide a quick therapeutic option to treat COVID-19. Communicated by Ramaswamy H. Sarma.
Journal of Biomolecular Structure and Dynamics published new progress about COVID-19. 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Computed Properties of 58-97-9.
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem