Ryu, Incheol; Kim, Yoon Ki published the artcile< AU-rich element-mediated mRNA decay via the butyrate response factor 1 controls cellular levels of polyadenylated replication-dependent histone mRNAs>, Formula: C9H13N2O9P, the main research area is mRNA decay AU rich element BRF1 polyadenylated replication histone; ARE-mediated mRNA decay; ELAV like RNA binding protein 1 (ELAVL1); Hu antigen R (HuR); RNA; RNA degradation; RNA metabolism; RNA turnover; RNA-protein interaction; ZFP36 ring finger protein like 1 (ZFP36L1); butyrate response factor 1 (BRF1); histone mRNA biogenesis; mRNA stability; polyadenylation; posttranscriptional regulation; replication-dependent histone mRNA.
Replication-dependent histone (RDH) mRNAs have a nonpolyadenylated 3′-UTR that ends in a highly conserved stem-loop structure. Nonetheless, a subset of RDH mRNAs has a poly(A) tail under physiol. conditions. The biol. meaning of poly(A)-containing (+) RDH mRNAs and details of their biosynthesis remain elusive. Here, using HeLa cells and Western blotting, qRT-PCR, and biotinylated RNA pulldown assays, we show that poly(A)+ RDH mRNAs are post-transcriptionally regulated via adenylate- and uridylate-rich element-mediated mRNA decay (AMD). We observed that the rapid degradation of poly(A)+ RDH mRNA is driven by butyrate response factor 1 (BRF1; also known as ZFP36 ring finger protein-like 1) under normal conditions. Conversely, cellular stresses such as UV C irradiation promoted BRF1 degradation, increased the association of Hu antigen R (HuR; also known as ELAV-like RNA-binding protein 1) with the 3′-UTR of poly(A)+ RDH mRNAs, and eventually stabilized the poly(A)+ RDH mRNAs. Collectively, our results provide evidence that AMD surveils poly(A)+ RDH mRNAs via BRF1-mediated degradation under physiol. conditions.
Journal of Biological Chemistry published new progress about 3′-Untranslated region Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 58-97-9 belongs to class tetrahydrofurans, and the molecular formula is C9H13N2O9P, Formula: C9H13N2O9P.
Referemce:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem