The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Alkylation of nucleic acids. I. Comparison study of the methylation of ribonucleic acids in aqueous and organic solutions》. Authors are Bollack, C.; Keith, G.; Ebel, J. P..The article about the compound:8-Bromoguaninecas:3066-84-0,SMILESS:NC(N1)=NC(NC(Br)=N2)=C2C1=O).Electric Literature of C5H4BrN5O. Through the article, more information about this compound (cas:3066-84-0) is conveyed.
Methylation of ribosomal and soluble RNA (from bakers’ yeast) by Me2SO4 in dimethylformamide (I) solution gives rise to the same derivatives as the methylation in aqueous solution, namely, 7-methylguanine, 1-methyladenine, and 1-methylcytosine. Uracil is not methylated. The percentages of methylated bases formed are lower in I medium than in water. Differences in the order of reactivity of the bases are observed according to the reaction carried out in I or in water. Methylation in aqueous solution does not affect the integrity of the RNA mols., even for high methylation percentages; but, methylation in I causes a degradation of the polynucleotide chains, especially for ribosomal RNA. Methylation causes some alteration of the secondary structure of soluble RNA and this alteration increases as the methylation percentage increases.
This compound(8-Bromoguanine)Electric Literature of C5H4BrN5O was discussed at the molecular level, the effects of temperature and reaction time on the properties of the compound were discussed, and the optimum reaction conditions were selected.
Reference:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem