HPLC of Formula: 3066-84-0. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: 8-Bromoguanine, is researched, Molecular C5H4BrN5O, CAS is 3066-84-0, about Flipping duplex DNA inside out: A double base-flipping reaction mechanism by Escherichia coli MutY adenine glycosylase. Author is Bernards, Andrew S.; Miller, Jamie K.; Bao, Kogan K.; Wong, Isaac.
The Escherichia coli MutY adenine glycosylase plays a critical role in repairing mismatches in DNA between adenine and the oxidatively damaged guanine base 8-oxoguanine. Crystallog. studies of the catalytic core domain of MutY show that the scissile adenine is extruded from the DNA helix to be bound in the active site of the enzyme (Guan, Y., Manuel, R. C., Arvai, A. S., Parikh, S. S., Mol, C. D., Miller, J. H., Lloyd, S., and Tainer, J. A. (1998) Nat. Struct. Biol. 5, 1058-1064). However, the structural and mechanistic bases for the recognition of the 8-oxoguanine remain poorly understood. In experiments using a single-stranded 8-bromoguanine-containing synthetic oligodeoxyribonucleotide alone and in a duplex construct mismatched to an adenine, we observed UV crosslinking between MutY and the 8-bromoguanine probe. We further observed enhanced crosslinking in the single strand experiments, suggesting that neither the duplex context nor the mismatch with adenine is required for recognition of the 8-oxoguanine moiety. Stopped-flow fluorescence studies using 2-aminopurine-containing oligodeoxyribonucleotides further revealed the sequential extrusion of the 8-oxoguanine at 108 s-1 followed by the adenine at 16 s-1. A protein isomerization step following base flipping at 1.9 s-1 was also observed and is postulated to provide addnl. stabilization of the extruded adenine thereby facilitating its capture by the active site for excision.
Different reactions of this compound(8-Bromoguanine)HPLC of Formula: 3066-84-0 require different conditions, so the reaction conditions are very important.
Reference:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem