Recommanded Product: 1-Hexyl-3,7-dimethyl-1H-purine-2,6(3H,7H)-dione. The protonation of heteroatoms in aromatic heterocycles can be divided into two categories: lone pairs of electrons are in the aromatic ring conjugated system; and lone pairs of electrons do not participate. Compound: 1-Hexyl-3,7-dimethyl-1H-purine-2,6(3H,7H)-dione, is researched, Molecular C13H20N4O2, CAS is 1028-33-7, about Active Site Crowding of Cytochrome P450 3A4 as a Strategy To Alter Its Selectivity. Author is Schiavini, Paolo; Cheong, Kin J.; Moitessier, Nicolas; Auclair, Karine.
Substrate-promiscuous enzymes are a promising starting point for the development of versatile biocatalysts. In this study, human cytochrome P 450 3A4, known for its ability to metabolize hundreds of drugs, was engineered to alter its regio- and stereoselectivity. Rational mutagenesis was used to introduce steric hindrance in a specific manner in the large active site of P 450 3A4 and to favor oxidation at a more sterically accessible position on the substrate. Hydroxylation of a synthetic precursor of (R)-lisofylline, a compound under investigation for its anti-inflammatory properties, was chosen as a first proof-of-principle application of our protein engineering strategy. In a second example, increasing active site crowding led to an incremental shift in the selectivity of oxidation from an internal double bond to a terminal Ph group in a derivative of theobromine. The same correlation between crowding and selectivity was found in a final case focused on the hydroxylation of the steroid sex hormone progesterone.
Different reactions of this compound(1-Hexyl-3,7-dimethyl-1H-purine-2,6(3H,7H)-dione)Recommanded Product: 1-Hexyl-3,7-dimethyl-1H-purine-2,6(3H,7H)-dione require different conditions, so the reaction conditions are very important.
Reference:
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem