Application of 17347-61-4, Chemistry is the science of change. But why do chemical reactions take place? Why do chemicals react with each other? The answer is in thermodynamics and kinetics.In a document type is Article, and a compound is mentioned, 17347-61-4, 2,2-Dimethylsuccinicanhydride, introducing its new discovery.
Chemical probing of the human sirtuin 5 active site reveals its substrate acyl specificity and peptide-based inhibitors
Sirtuins are NAD+-dependent deacetylases acting as sensors in metabolic pathways and stress response. In mammals there are seven isoforms. The mitochondrial sirtuin 5 is a weak deacetylase but a very efficient demalonylase and desuccinylase; however, its substrate acyl specificity has not been systematically analyzed. Herein, we investigated a carbamoyl phosphate synthetase 1 derived peptide substrate and modified the lysine side chain systematically to determine the acyl specificity of Sirt5. From that point we designed six potent peptide-based inhibitors that interact with the NAD+ binding pocket. To characterize the interaction details causing the different substrate and inhibition properties we report several X-ray crystal structures of Sirt5 complexed with these peptides. Our results reveal the Sirt5 acyl selectivity and its molecular basis and enable the design of inhibitors for Sirt5. Small changes make the difference: Systematic investigation of the acyl substrate specificity of sitruin 5 uncovers its unique preference for succinyl and glutaryl residues. Depending on the chemical nature of introduced modifications, either high-affinity substrates or sirtuin 5 specific inhibitors could be obtained.
Balanced chemical reaction does not necessarily reveal either the individual elementary reactions by which a reaction occurs or its rate law.Application of 17347-61-4. In my other articles, you can also check out more blogs about 17347-61-4
Reference£º
Tetrahydrofuran – Wikipedia,
Tetrahydrofuran | (CH2)3CH2O – PubChem